请问fish检测有必要重复做么？

大v

我父亲之前在长沙确诊为多发性骨髓瘤的时候已经做了fish检测，现在到北京我这里来住一段时间，到医院去做复查的时候医生说离上一次做有一年的时间了，建议我们再做一次。我感到很疑惑，这个FISH检测结果难道还会老变吗？有必要再做吗？

原文地址：https://www.zhihu.com/question/354860212

感恩由您

Probe of FISH-quant

• Reference paper
  

• Download R script
  

https://bitbucket.org/muellerflorian/fish_quant/src/master/Oligostan/Oligostan.r
If you want to use " Use of RepeatMasker Filter ", the Linux platform may be as well. MAC that so expensive that I cannot use it.

• RepeatMasker
  

wget http://www.repeatmasker.org/RepeatMasker/RepeatMasker-4.1.2-p1.tar.gz

• rmblastn
  

wget ftp://ftp.ncbi.nlm.nih.gov/blast/executables/rmblast/2.2.28/ncbi-rmblastn-2.2.28-src.tar.gz

file error  by  wget and solve it with curl -o xxx url.

curl -o daf ftp://ftp.ncbi.nlm.nih.gov/blast/executables/rmblast/2.2.28/ncbi-rmblastn-2.2.28-src.tar.gz
tar xzvf ncbi-rmblastn-2.2.28-src.tar.gz
tar xzvf RepeatMasker-4.1.2-p1.tar.gz

• Install
  

cd RepeatMasker
chmod -R 777 *
./configure

Remember to download TRF software and filed with fill path

https://objects.githubusercontent.com/github-production-release-asset-2e65be/253959277/08d4c580-b9e6-11ea-96c1-2c1b402554d5?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIAIWNJYAX4CSVEH53A%2F20220426%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Date=20220426T040238Z&X-Amz-Expires=300&X-Amz-Signature=f4a471086d00ffc2460e5e47575a3f0555026d8226c7795a30d05f5751bea68f&X-Amz-SignedHeaders=host&actor_id=0&key_id=0&repo_id=253959277&response-content-disposition=attachment%3B%20filename%3Dtrf409.linux64&response-content-type=application%2Foctet-stream

./configure
## filed with this path
{your path}/trf409.linux64

select 2 to configure and then YES until end.
install R packages

install.packages ("zoo","ade4","seqinr")

• [ ] > install python module
  

Resolve above all and configure again


That‘s all
**
Remember to update HMMER & DFAM so that your filter more specific  OR you can use blast to align with your reference genome to filter.**

同花顺

原则上不需要。但如果结果是不确定阳性，需要结合免疫组化检测结果综合判断。那么，换家医院重做一次就是必要的。

同花顺

会变，我们家病人一共做了3次fish，每次结果都不同，因为fish不光会提示是基因的什么问题，还会提示比例，例如基因缺失/重排/错位百分之多少，经过化疗后，可能是会有修复的（不知道医学上是不是用这个词）

感恩由您

补充一下，在长沙已经做了五个疗程的化疗，现在病情基本稳定，吃来那杜胺维持治疗，这次只是来北京小住，去医院做定期复查的时候医生提出的。